Web15 Oct 2024 · Peak distortions are a common and frustrating problem when performing chromatographic experiments, and peak tailing in high-performance liquid chromatography (HPLC) is one of the most frequent distortions that can occur. One of the challenging … WebLow sensitivity and rising baselines, noise, or spikes on the chromatogram can often be attributed to the mobile phase. Contaminants in the mobile phase are especially troublesome in gradient elution. The baseline may rise, and spurious peaks can appear as the level of the contaminated component increases.
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Web12 May 2024 · How to avoid peak tailing: 1. Operate at a lower pH As silanol groups are acidic, secondary interactions can be minimized by performing the chromatographic separation at a lower pH — thereby... WebPeak Tailing Identifying Column “Secondary Interactions” Tip: Mobile phase modifier (TEA) competes with Sample for surface ion exchange sites at mid-range pH values Column: Alkyl-C8, 4.6 x 150 mm, 5μm Mobile Phase: 85% 25 mM Na 2HPO 4pH 7.0 : 15% ACN Flow Rate: 1.0 mL/min Temperature: 35°C Sample: 1. diseases associated with cholesterol
LABTips: How to Prevent Tailing Peaks in HPLC - Labcompare
WebThe tailing would occur for all analytes and is likely to be accompanied by a change in column pressure. A column with this type of damage should be replaced. Loss of inertness in tubing, injector parts or associated valves- If switching to a new column doesn’t help, it … WebTailing peak is related to the column, maybe overload, dilute the sample, or use a new column. Sometimes the tailing peak is caused by the unseparated impurities with similar organic properties. You may need to optimize the analysis method or replace the column. Web1 Dec 2013 · Tailing is most commonly attributed to unwanted secondary interactions between the analyte molecule and the column. Usually this results from strong interactions between acidic silanol groups on the column packing and … diseases associated with cigarette smoking