Tg gata1a:dsred

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August undefined, 2024

WebTg (gata1:dsRed)sd2 ( 1) Affected Genomic Region. This feature is representative of one or more unknown insertion sites. Construct. Tg (gata1a:DsRed) Type. Transgenic insertion ( 1 ) Protocol. embryos treated with DNA. Web9 mag 2014 · (C) Bright-field (DIC) and epifluorescent (GFP and DsRED) micrographs of Tg(kdrl:GFP) s843 /Tg(gata1:DsRED) sd2 embryos at 72 hpf that were injected with control, gata1a or gata2a morpholinos.

Hif-1α regulates macrophage-endothelial interactions during blood ...

WebConfocal imaging of 3.5 dpf Tg(fli1: EGFP y1 ;gata1a:dsRed sd2 ) ( Yaniv et al., 2006) embryos revealed the presence of rostrocaudal blood flow in the SIA, whereas circulation in the SIV followed ... Web19 mag 2024 · To determine whether these mutants exhibit vascular defects, hif-1aa bns89 and hif-1ab bns90 were crossed into the Tg(kdrl:EGFP) s843;Tg(gata1a:DsRed) sd2 … shoulder m75.40 disability rating

Blood Flow Changes Coincide with Cellular Rearrangements …

Web120 Tg(gata1a:DsRed) 26. Tg(ins:cdt1-mCherry) was utilised in preference of a pan β cell marker 121 such as Tg(ins:mKO-nls) to allow for a clear separation of the spectra and simultaneous 122 signal recordings from the GCaMP and mCherry channels, which was particularly important Web10 feb 2024 · Tg(fli1a:EGFP) transgenic embryos at 48 hpf for vascular monitor were immobilized in 1% low melting point agarose containing 0.02% MS-222 and imaged with Zeiss710 or Zeiss710 META confocal microscope. Tg(kdrl:GFP;gata1a:DsRed) embryos were used for time lapse imaging from 38 to 48 hpf under Olympus FV1200 confocal … WebTg2 (gata1:DsRed) ( 1) Type engineered_plasmid Regulatory Regions gata1a ( 1) Coding Sequences DsRed ( 1) [Em λ] [Ex λ] 583 (red) 558 (orange) Fpbase:DsRed Contains … shoulder lysis of adhesions

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Tg gata1a:dsred

ZFIN Feature: sd2Tg - Zebrafish Information Network

WebA Blood circulation in the intersegmental vessels of the control (A1–A3) and foxm1 MO injected (A4–A6) Tg(fli1a:GFP; gata1:dsRed) zebrafish larvae at 72 hpf. “1, 2, 3,” … Web1 apr 2024 · In this study, Tg (drl: GFP) embryos at 24 hpf and Tg (gata1a: DsRed) embryos at 24/48/72 hpf were collected and fixed in 4% paraformaldehyde overnight, …

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Web26 gen 2024 · d Simultaneously acquired TSFG and fluorescence images of RBCs in a 2 dpf Tg(gata1a:DsRed) embryo. In this experiment, we induced transient cardioplegia using … Web1 mag 2014 · Pang et al. (2024) used the double transgenic Tg(kdrl: EGFP; gata1a: dsRed) to assess erythropoiesis and circulatory anomalies in embryos exposed to tetrabromobisphenol A (TBBPA).

Web1 feb 2024 · By using Tg(fli1a:EGFP)y1 & Tg(gata1a:dsRed)sd2 homozygous double transgenic zebrafish to visualize the endothelial cells (green fluorescence) and erythrocytes (red fluorescence) in vivo, here we showed that the pre-treatment of Tan I was effective in reducing the rate and size of atorvastatin-induced cerebral hemorrhage (Fig. 2, Fig ... Web19 mag 2024 · To determine whether these mutants exhibit vascular defects, hif-1aa bns89 and hif-1ab bns90 were crossed into the Tg(kdrl:EGFP) s843;Tg(gata1a:DsRed) sd2 lines, hereafter Tg(kdrl:EGFP);Tg(gata1 ...

Web2 set 2024 · The line of Tg(gata1a: dsRed) was used in this study. After the generation of vgll4b mutant zebrafish was achieved using CRISPR/Cas9, the genotyping PCR primers were designed as follows: forward 5′-GGTGAACCAGCTGAAAGCTGTAACC-3’; reverse 5′-CTTTATCCGCCGAGATGTTGAAAG-3’. Web1 gen 2024 · The vgll4b-/-Tg(gata1a: dsRed) zebrafish maintenance was then established and used. 2.2. Plasmid construction. Zebrafish genes, and mutants, were amplified from reverse transcription products and inserted into the tagged PCS2 + vector with their respective primers.

Web23 apr 2024 · The decrease in gata1a expression was consistent with a decrease in the number of Gata1 + cells as demonstrated by the Tg(gata1a:dsRed) (Figure 5I, P = 2.7125e-07). Collectively, these data suggest that isoprenoids regulate RBC differentiation in a gata1a dependent manner. Download : Download high-res image (282KB)

Web25 mag 2024 · Finally, the lymphatic versus blood vessel identity was functionally confirmed through time-lapse imaging of Tg (gata1a:dsRed) that showed erythrocyte flow only in … saslong classic clubWebZebrafish embryos expressing the fluorescent vascular marker Tg(kdrl:EGFP) in cyan and the red blood cell marker, Tg(GATA1a:dsRed) in magenta imaged from three different … sas london to stockholmWeb8 set 2024 · Since its debut in the biomedical research fields in 1981, zebrafish have been used as a vertebrate model organism in more than 40,000 biomedical research studies. Especially useful are zebrafish lines expressing fluorescent proteins in a molecule, intracellular organelle, cell or tissue specific manner because they allow the visualization … sas long to wide transpose